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Test Name:
Iron, Serum (Total)


  • TMF No:
    35300
  • Performance Lab Name:
    TMF Automated Laboratory / Hematology
  • Test Mnemonic:
    IRON-ONLY
  • ABN:
    National Coverage Determination (NCD) for Serum Iron Studies (190.18)
  • CPT Code:
    83540
  • Also See:
    29100 Iron & Iron Binding Capacity, Serum
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Alt Spec Type:
    Plasma
  • Alt Spec Container:
    Green top (lithium heparin) tube
  • Pref Vol:
    1.0 mL
  • Min Vol:
    0.3 mL
  • Patient Prep:
    Document on requisition if patient received iron therapy
    Document on requisition if patient received recent transfusion
  • Spec Collect:
    Routine venipuncture
    When possible, collect specimen prior to patient recovering therapeutic iron transfusion
    Wait at least 4 days after any transfusion to collect specimen
  • Spec Process:
    Serum sample tubes, clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum or plasma to separate plastic tube
  • Spec Store Transport:
    Refrigerated.
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Spec Reject:
    Hemolyzed sample
  • Methodology:
    Photometric
  • Use:
    Used to diagnosis and treatment of diseases such as iron deficiency anemia, hemochromatosis (a disease associated with widespread deposit in the tissue of the two iron-containing pigments, hemosiderin and hemofuscin, and characterized by pigmentation of skin), and chronic renal disease
  • Clinical Significance:
    Ingested iron is mainly absorbed in the form of Fe2+ in the duodenum and upper jejunum. The trivalent form and the heme-bound Fe2+-component of iron in food have to be reduced by vitamin C. About 1 mg of iron is assimilated daily. Upon reaching the mucosal cells, Fe2+ ions become bound to transport substances. Before passing into the plasma, these are oxidized by ceruloplasmin to Fe3+ and bound to transferrin in this form. The transport of Fe ions in blood plasma takes place via transferrin-iron complexes. A maximum of 2 Fe2+ ions per protein molecule can be transported. Serum iron is almost completely bound to transferrin.Iron determinations are performed for the diagnosis and monitoring of microcytic anemia (e.g. due to iron metabolism disorders and hemoglobinopathy), macrocytic anemia (e.g. due to vitamin B12 deficiency, folic acid deficiency and drug-induced metabolic disorders of unknown origin) as well as normocytic anemias such as renal anemia (erythropoetin deficiency), hemolytic anemia, hemoglobinopathy, bone marrow disease and toxic bone marrow damage.
  • Additional Test Info:
    Lab Tests Online
  • Day Run:
    Sun-Sat
  • Time Run:
    As received
  • Time Reported:
    4 hours
  • Test Type:
    METAL/ELEMENT