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Test Name:
IgE, Quantitative, Serum


  • TMF No:
    30099
  • Performance Lab Name:
    TMF Chemistry and Immunoassays
  • Test Mnemonic:
    IGE
  • ABN:
    Local Coverage Determination (LCD): Allergy Testing (L36402)
  • CPT Code:
    82785
  • Also Known As:
    Immunoglobulin E
  • Spec Type:
    Serum
  • Spec Container:
    Gold top (SST) or red top (serum) tube
  • Alt Spec Type:
    Plasma
  • Alt Spec Container:
    Green top (sodium or lithium heparin) or Lavender top (EDTA) tube
  • Pref Vol:
    1.0 mL
  • Min Vol:
    0.3 mL
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Clot 30 minutes
    Promptly centrifuge 15 minutes
    Immediately transfer serum to separate plastic tube
    Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube
  • Spec Store Transport:
    Refrigerated.
  • Spec Stability:
    24 hours room temperature (20-30°C)
    1 week refrigerated (2-8°C)
    Greater than 1 week, freeze (-20°C) (avoid repeated freeze/thaw cycles)
  • Methodology:
    ImmunoCAP™ Fluorescent Enzyme Immunoassay (FEIA)
  • Use:
    Diagnose and manage patients with atopic disease such as asthma, hay fever, eczema, and patients with respiratory allergies or parasitic infections
  • Clinical Significance:
    Immunoglobulin E (IgE) is a class of immunoglobulins important in the mediation of allergic responses. IgE is synthesized primarily within plasma cells found in the mucosa of the respiratory and intestinal tracts and is secreted into the blood. The mechanism of action of the allergic response involves the binding of IgE through its Fc portion to cell surface receptors of mast cells and basophils. After exposure to specific allergens recognized by IgE, the cells release vasoactive amines causing Type I hypersensitivity reactions. IgE levels may be increased in not only allergic disease, but also parasitic infections and bronchopulmonary aspergillosis.
  • Day Run:
    Mon, Tue, Thu, Fri
  • Time Run:
    8:00 am
  • Time Reported:
    5:00 pm
  • Test Type:
    IMMUNOLOGY